Chemical Identity
| Name | Thymosin Beta-4 (TB-500) |
|---|---|
| Synonyms | Thymosin β4, Tβ4, Timbetasin |
| CAS Number | 77591-33-4 |
| Molecular Formula | C212H350N56O78S |
| Molecular Weight | 4963.44 g/mol |
| Sequence | Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH |
| Structure Class | Full-length 43-amino-acid N-terminally acetylated peptide. Note: some suppliers use "TB-500" for the Ac-LKKTETQ (17–23) fragment; this product is the full-length sequence. |
Mechanisms at a Glance
- Binds monomeric G-actin 1:1 through the LKKTETQ motif (residues 17–23)
- Regulates the actin polymerization that underlies cell motility
- Forms a complex with PINCH and integrin-linked kinase, activating Akt in cardiac models
Research Applications
- Cell migration and wound-closure assays
- Angiogenesis models
- Cardiac, corneal and hair-follicle research
Handling & Storage
Store lyophilized material at −20 °C, desiccated and protected from light; after reconstitution, keep solutions at 2–8 °C and use within 28 days. See our storage and stability guide and reconstitution guide for technique and diluent selection.
Regulatory Status
Thymosin beta-4 is not approved by the FDA or any regulatory agency for human use. Material supplied by Aclopiel is strictly for in-vitro laboratory research and is not for human or veterinary use.
Frequently Asked Questions
What is TB-500?
TB-500 is the research name for synthetic Thymosin Beta-4, a 43-residue N-acetylated peptide that is the principal G-actin-sequestering protein in mammalian cells.
What is the CAS number of TB-500?
TB-500 is registered under CAS number 77591-33-4. Its molecular formula is C212H350N56O78S and its molecular weight is 4963.44 g/mol.
How should TB-500 be stored?
Store lyophilized TB-500 at −20 °C, desiccated and protected from light. After reconstitution, keep it at 2–8 °C and use it within 28 days.
Is TB-500 approved for human use?
Thymosin beta-4 is not approved by the FDA or any regulatory agency for human use. Aclopiel supplies it strictly for in-vitro laboratory research; it is not for human or veterinary use.
Is TB-500 the same as Thymosin Beta-4?
TB-500 is a research designation. Some suppliers use it for full-length Thymosin β4 (43 residues, CAS 77591-33-4); others for the Ac-LKKTETQ fragment. Our material is the full-length sequence.
What is the LKKTETQ motif?
Residues 17–23 of Thymosin β4 form its central actin-binding region and are the basis of shorter fragment analogues.
Alternative Designations & Synonyms
TB-500 also appears in the literature and supplier listings under the following names and codes:
- Thymosin β4
- Tβ4
- Timbetasin
- TB 500
- TB500
- Thymosin Beta-4
- Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES
References
Malinda KM et al., J Invest Dermatol 1999; Bock-Marquette I et al., Nature 2004; Goldstein AL et al., Expert Opin Biol Ther 2012; Philp D et al., FASEB J 2004.