Chemical Identity
| Name | 5-Amino-1-methylquinolinium |
|---|---|
| Synonyms | 5-amino-1MQ, NNMTi |
| CAS Number | 685079-15-6 |
| Molecular Formula | C10H11N2+ |
| Molecular Weight | 159.21 g/mol |
| Sequence | 5-amino-1-methylquinolin-1-ium iodide |
| Structure Class | Methylated quinolinium cation; substrate-site nicotinamide N-methyltransferase (NNMT) inhibitor |
Mechanisms at a Glance
- Substrate-site-directed NNMT inhibitor, with a reported IC50 around 1.2 µM
- NNMT methylates nicotinamide to 1-methylnicotinamide using SAM as the methyl donor, diverting nicotinamide away from NAD+ salvage
- Inhibiting the enzyme is therefore studied as a route to preserving NAD+ precursor availability — the mechanistic link to the NAD+ literature
- Reported to reduce lipogenesis in 3T3-L1 adipocyte culture with an EC50 near 30 µM, roughly 25-fold above its enzymatic IC50
Research Applications
- NNMT enzyme-inhibition assays
- NAD+ salvage-pathway studies
- Adipocyte differentiation and lipogenesis models
Handling & Storage
Store lyophilized material at −20 °C, desiccated and protected from light; after reconstitution, keep solutions at 2–8 °C and use within 28 days. Store at −20 °C, protected from light. Prepare a DMSO stock; account for the iodide counter-ion in molar calculations. See our storage and stability guide and reconstitution guide for technique and diluent selection.
Regulatory Status
5-Amino-1MQ is a research chemical and is not approved by the FDA as a drug for any indication. Material supplied by Aclopiel is strictly for in-vitro laboratory research and is not for human or veterinary use.
Frequently Asked Questions
What is 5-Amino-1MQ?
5-Amino-1MQ is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT). It is a methylated quinolinium compound, not a peptide, and it sits in the same NAD+ literature as the cofactor itself.
What is the CAS number of 5-Amino-1MQ?
5-Amino-1MQ is registered under CAS number 685079-15-6. Its molecular formula is C10H11N2+ and its molecular weight is 159.21 g/mol.
How should 5-Amino-1MQ be stored?
Store lyophilized 5-Amino-1MQ at −20 °C, desiccated and protected from light. After reconstitution, keep it at 2–8 °C and use it within 28 days. Store at −20 °C, protected from light. Prepare a DMSO stock; account for the iodide counter-ion in molar calculations.
Is 5-Amino-1MQ approved for human use?
5-Amino-1MQ is a research chemical and is not approved by the FDA as a drug for any indication. Aclopiel supplies it strictly for in-vitro laboratory research; it is not for human or veterinary use.
Which CAS number and mass are correct?
The certificate identifies the free cation: CAS 685079-15-6, C10H11N2+, 159.21 g/mol. The iodide salt is sometimes listed instead as CAS 42464-96-0 at 286.11 g/mol. About 1.89 mg of the iodide salt delivers 1 mg of the free cation, so molar calculations that ignore the counter-ion overstate the active species by nearly a factor of two.
How is it dissolved?
As a DMSO stock, diluted into assay buffer. Solubility in DMSO is around 2 mg/mL; it is not a bacteriostatic-water compound.
Is it related to NAD+?
Mechanistically, yes. NNMT consumes nicotinamide, which is the substrate NAD+ salvage depends on, so inhibiting NNMT is studied as a way of preserving NAD+ precursor supply. The two compounds are frequently used together.
Alternative Designations & Synonyms
5-Amino-1MQ also appears in the literature and supplier listings under the following names and codes:
- NNMTi
- 5-amino-1-methylquinolinium
- PubChem CID 66522933
References
Neelakantan H et al., Biochem Pharmacol 2017;147:141-152; Neelakantan H et al., J Med Chem 2017;60:5015-5028; Sigma-Aldrich product SML2832 technical data.