Lab Technique
The Researcher's Guide to Reconstitution Ratios and Bacteriostatic Calculations
Diluent selection, concentration maths and handling technique for turning a lyophilized peptide cake into an accurate, stable working solution.
Tissue Repair · Lyophilized Powder
TB-500 (Thymosin Beta-4) Research Peptide - 10mg Vial
CAS: 77591-33-4 | Sequence: 43-aa N-acetylated peptide | Purity: ≥99.1%
$69.99 / vial
In Stock - Ships Within 24 Hours
- Cold Storage −20 °C lyophilized
- Reconstituted 2–8 °C · 28 days
- HPLC ≥99.1% third-party verified
SKU PT-TB500-10 · HPLC + MS tested every batch · Insulated cold-pack shipping
What Is TB-500?
TB-500 is the research designation commonly used for synthetic Thymosin Beta-4 (Tβ4), a 43-amino-acid, N-terminally acetylated peptide (4963.44 g/mol) encoded by the human TMSB4X gene. Tβ4 is one of the most abundant intracellular peptides in mammalian cells and the principal regulator of the monomeric (G-) actin pool.
Because actin dynamics underpin cell shape and motility, Tβ4 has been investigated in models of cell migration, wound closure, angiogenesis, cardiac injury, corneal repair and hair-follicle activity. Its central LKKTETQ actin-binding motif (residues 17–23) has also been synthesized as a shorter fragment, which some suppliers sell under the same "TB-500" name.
This product is the full-length 43-residue sequence (CAS 77591-33-4), supplied as 10 mg of lyophilized powder at ≥99.1% HPLC purity with a batch-specific third-party COA.
Third-Party Certificate of Analysis
Each production lot is independently tested for identity, purity and content. Select a batch to view its analytical results — your vial label lists the batch number.
HPLC purity (UV 220 nm)
99.32%
Tested by: —
| Test | Method | Result | Status |
|---|
TB-500 Scientific Documentation
Technical reference data and a summary of published preclinical literature, provided for educational purposes.
| Name | Thymosin Beta-4 (TB-500) |
|---|---|
| Synonyms | Thymosin β4, Tβ4, Timbetasin |
| CAS Number | 77591-33-4 |
| Molecular Formula | C212H350N56O78S |
| Molecular Weight | 4963.44 g/mol |
| Sequence (3-letter) | Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH |
| Sequence (1-letter) | Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES-OH |
| Structure Class | Full-length 43-amino-acid N-terminally acetylated peptide. Note: some suppliers use "TB-500" for the Ac-LKKTETQ (17–23) fragment; this product is the full-length sequence. |
| Form | White lyophilized powder |
| Purity | ≥99.1% (RP-HPLC); identity confirmed by ESI-MS |
| Content per Vial | 10 mg net peptide |
| Solubility | Soluble in water, bacteriostatic water and 0.9% saline |
| Storage Conditions | Lyophilized: −20 °C, desiccated and protected from light. Reconstituted: 2–8 °C, use within 28 days. |
The findings below come from in-vitro and animal studies. Thymosin beta-4 is not approved by the FDA or any regulatory agency for human use, and nothing on this page is medical advice or a therapeutic claim.
G-Actin Sequestration
Thymosin β4 is the principal G-actin–sequestering peptide in mammalian cells, binding monomeric actin in a 1:1 complex and regulating cytoskeletal polymerization. The central LKKTETQ motif (residues 17–23) forms the actin-binding region and is the basis of fragment analogues.
Cell Migration & Wound Models
In rat full-thickness wound models, topical and intraperitoneal Tβ4 was associated with increased re-epithelialization, collagen deposition and angiogenesis, alongside enhanced keratinocyte and endothelial cell migration in vitro.
Cardiac Research
In a mouse myocardial infarction model, Tβ4 formed a functional complex with PINCH and integrin-linked kinase (ILK), activating Akt signalling and promoting cardiomyocyte survival and migration.
Anti-Inflammatory & Ocular Models
Corneal injury models have reported reduced inflammatory cytokine expression and faster epithelial closure following Tβ4 exposure.
Hair Follicle Research
In rodent studies, Tβ4 accelerated hair growth and was associated with migration and differentiation of hair-follicle stem cells and their progeny, linking its actin-regulating activity to follicle cycling.
Research Limitations
Much of the mechanistic data comes from rodent and cell-culture systems, and published studies differ in whether they used the full-length peptide or the LKKTETQ fragment, which complicates comparison.
Representative literature: Malinda KM et al., J Invest Dermatol 1999; Bock-Marquette I et al., Nature 2004; Goldstein AL et al., Expert Opin Biol Ther 2012; Philp D et al., FASEB J 2004.
Bacteriostatic Water Ratio Chart (10 mg Vial)
| Diluent Added | Concentration | Per 0.1 mL Aliquot |
|---|---|---|
| 1.0 mL | 10.0 mg/mL | 1 mg |
| 2.0 mL | 5.0 mg/mL | 500 µg |
| 2.5 mL | 4.0 mg/mL | 400 µg |
| 3.0 mL | 3.33 mg/mL | 333 µg |
| 5.0 mL | 2.0 mg/mL | 200 µg |
Reconstitution Procedure
Allow the vial to reach room temperature. Introduce sterile or bacteriostatic water slowly down the inner glass wall — do not inject directly onto the powder cake. Swirl gently until fully dissolved; do not shake or vortex, as this can promote aggregation.
Temperature Stability
| State | Condition | Typical Stability |
|---|---|---|
| Lyophilized | −20 °C, desiccated | 24+ months |
| Lyophilized | 2–8 °C | Up to 12 months |
| Lyophilized | Room temp (≤25 °C) | Several weeks (transit) |
| Reconstituted | 2–8 °C | Up to 28 days |
| Reconstituted | −20 °C aliquots | Avoid repeated freeze–thaw |
Further Reading
Research Review
Comparative Analysis of Tissue Regeneration Pathways: BPC-157 vs. TB-500 In-Vitro Models
Two of the most studied tissue-repair peptides work through very different mechanisms. A side-by-side look at pathways, model systems and study-design pitfalls.
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