Laboratory Tool
Peptide Reconstitution Calculator
Enter the peptide mass in your vial, the volume of bacteriostatic water added and the amount you need per aliquot. The calculator returns the concentration, the exact volume to measure and where it falls on a graduated 1 mL scale.
How the Calculation Works
Concentration (mg/mL) = peptide (mg) ÷ diluent (mL)
Volume (mL) = target (µg) ÷ (concentration × 1,000)
Because 1 mg/mL equals 1 µg/µL, the concentration also tells you how many micrograms are in each microlitre. Choose a diluent volume that puts your working aliquots on a convenient graduation — volumes between the 0.01 mL marks, or below 0.01 mL, are better measured with a calibrated micropipette.
Common Concentrations
| Diluent | 2 mg vial | 5 mg vial | 10 mg vial |
|---|---|---|---|
| 1.0 mL | 2 mg/mL | 5 mg/mL | 10 mg/mL |
| 2.0 mL | 1 mg/mL | 2.5 mg/mL | 5 mg/mL |
| 3.0 mL | 0.67 mg/mL | 1.67 mg/mL | 3.33 mg/mL |
| 5.0 mL | 0.4 mg/mL | 1 mg/mL | 2 mg/mL |
For diluent selection, technique and storage after reconstitution, read our reconstitution guide and storage and stability protocols.
Compounds That Need Extra Care
Acylated analogues such as semaglutide and tirzepatide self-associate more readily than short unmodified peptides, so they should be swirled rather than shaken and moved to cold storage promptly after reconstitution. Our semaglutide vs. tirzepatide comparison covers the handling differences between them, and each product page lists compound-specific notes alongside its ratio chart.
Frequently Asked Questions
How is the volume calculated?
Concentration (mg/mL) is peptide mass divided by diluent volume. The volume to measure is the target amount in micrograms divided by the concentration × 1,000 — for example, 250 µg from a 5 mg vial in 2 mL (2.5 mg/mL) is 0.100 mL.
Why is the result shown in millilitres?
Laboratory measurements are made in volume units. The scale shows 0.01 mL graduations, so the result can be read directly on a 1 mL graduated syringe or pipette, or converted to µL for a micropipette.
Does this calculator give dosing advice?
No. It is a laboratory aliquoting tool. Aclopiel compounds are sold strictly for in-vitro research, and we do not provide dosing or human-use guidance.