Longevity · Lyophilized Powder
NAD+ (β-Nicotinamide Adenine Dinucleotide) - 500mg Vial
CAS: 53-84-9 | Sequence: Pyridine dinucleotide cofactor | Purity: ≥98.5%
74.99 / vial
Pre-Order - First Batch in Final QC
- Cold Storage −20 °C lyophilized
- Reconstituted 2–8 °C · 28 days
- HPLC ≥98.5% third-party verified
SKU PT-NAD-500 · HPLC + MS tested every batch · Insulated cold-pack shipping
What Is NAD+?
β-Nicotinamide adenine dinucleotide (NAD+) is the redox cofactor at the centre of cellular energy metabolism — a dinucleotide of 663.43 g/mol, not a peptide. It is supplied here as a reference-grade reagent because it is routinely used alongside peptide work in metabolic and mitochondrial assays.
NAD+ plays two distinct roles. As a cofactor it cycles reversibly between NAD+ and NADH, carrying electrons through glycolysis and oxidative phosphorylation. As a substrate it is irreversibly consumed by sirtuins, PARPs and CD38, which cleave it to release nicotinamide — and that consumption is why NAD+ availability became a question in ageing biology at all.
Supplied as 500 mg of hygroscopic white to off-white powder at ≥98% HPLC purity with a batch-specific third-party COA. Store desiccated and prepare solutions fresh. The first production batch is in final QC; the form below will bring you the certificate.
Request the Full HPLC Report
The first production batch of NAD+ is in final QC. Ask for the complete HPLC and mass-spec package and we will email it the moment the certificate is released.
For laboratory research use only. We do not provide dosing guidance or medical advice.
NAD+ Scientific Documentation
Technical reference data and a summary of published preclinical literature, provided for educational purposes.
| Name | β-Nicotinamide adenine dinucleotide (NAD+) |
|---|---|
| Synonyms | β-NAD, NAD, Coenzyme I, DPN (diphosphopyridine nucleotide) |
| CAS Number | 53-84-9 |
| Molecular Formula | C21H27N7O14P2 |
| Molecular Weight | 663.43 g/mol |
| Sequence (3-letter) | Nicotinamide mononucleotide–pyrophosphate–adenosine monophosphate |
| Sequence (1-letter) | Not applicable — dinucleotide, not a peptideNAD+ is a dinucleotide cofactor with no amino-acid sequence. It is listed here because researchers buy it alongside peptides, not because it is one. |
| Structure Class | Pyridine dinucleotide: nicotinamide riboside and adenosine linked by a pyrophosphate bridge — a small molecule, not a peptide |
| Form | White to off-white hygroscopic powder |
| Purity | ≥98.5% (RP-HPLC); identity confirmed by LC-MS |
| Content per Vial | 500 mg net peptide |
| Solubility | Freely soluble in water; prepare solutions fresh and keep cold, as NAD+ hydrolyses in aqueous solution |
| Storage Conditions | Lyophilized: −20 °C, desiccated and protected from light. Reconstituted: 2–8 °C, use within 28 days. NAD+ is hygroscopic and hydrolyses in solution; keep the vial sealed and desiccated, and prepare aqueous solutions immediately before use rather than storing them. |
What NAD+ Actually Does
NAD+ is the central redox cofactor of intermediary metabolism. It accepts a hydride to become NADH and is re-oxidized back to NAD+, shuttling electrons through glycolysis, the TCA cycle and oxidative phosphorylation. This is textbook biochemistry, not a research hypothesis.
Beyond Redox: Consuming Enzymes
NAD+ is also a substrate — not just a cofactor — for sirtuins, PARPs and CD38. These enzymes cleave the nicotinamide–ribose bond, which means they consume NAD+ stoichiometrically. This consumption is the mechanistic basis for most of the interest in NAD+ within ageing research.
Why It Is Studied in Ageing
Tissue NAD+ concentrations have been reported to decline with age across several species, and restoring them in model organisms has been associated with changes in mitochondrial function and sirtuin activity. Whether the decline is causal or consequential is still debated.
Handling and Stability
NAD+ is hygroscopic and unstable in aqueous solution, particularly at alkaline pH. Solutions should be made fresh, kept on ice and used promptly; the oxidized form also degrades on repeated freeze–thaw. The powder must be stored desiccated.
Limitations
Vendor literature frequently conflates NAD+ with its precursors NMN and NR, which have different pharmacokinetics and cannot be used interchangeably in experimental design. Confirm which species you need before ordering.
Representative literature: Covarrubias AJ et al., Nat Rev Mol Cell Biol 2021;22:119-141; Cantó C, Menzies KJ, Auwerx J, Cell Metab 2015;22:31-53; Yoshino J et al., Cell Metab 2018;27:513-528.
Bacteriostatic Water Ratio Chart (500 mg Vial)
| Diluent Added | Concentration | Per 0.1 mL Aliquot |
|---|---|---|
| 1.0 mL | 500.0 mg/mL | 50 mg |
| 2.0 mL | 250.0 mg/mL | 25 mg |
| 2.5 mL | 200.0 mg/mL | 20 mg |
| 3.0 mL | 166.67 mg/mL | 16.67 mg |
| 5.0 mL | 100.0 mg/mL | 10 mg |
Reconstitution Procedure
Allow the vial to reach room temperature. Introduce sterile or bacteriostatic water slowly down the inner glass wall — do not inject directly onto the powder cake. Swirl gently until fully dissolved; do not shake or vortex, as this can promote aggregation.
Temperature Stability
| State | Condition | Typical Stability |
|---|---|---|
| Lyophilized | −20 °C, desiccated | 24+ months |
| Lyophilized | 2–8 °C | Up to 12 months |
| Lyophilized | Room temp (≤25 °C) | Several weeks (transit) |
| Reconstituted | 2–8 °C | Up to 28 days |
| Reconstituted | −20 °C aliquots | Avoid repeated freeze–thaw |
Frequently Purchased Together
Complementary research compounds and lab supplies.